Unlockables are new content for the game such as items, characters, or challenges that must be unlocked. They pop up when something has been completed or achieved.

Name Image Description Unlock In Game Secret Number
Bethany
Achievement Bethany.png
Bethany
You unlocked Bethany Have any combination of 12 soul, black, or immortal hearts during a run 179
Jacob & Esau
Achievement Jacob & Esau.png
Jacob & Esau
You unlocked Jacob & Esau. Defeat The Witness 180
Planetarium
Achievement Planetarium.png
Planetarium
The stars are calling... Acquire three astrology-related items in one run. 181
A Secret Exit
Achievement A Secret Exit.png
A Secret Exit
Your fate diverges Defeat the three new bosses found on normal floors (Baby Plum, Reap Creep, and The Pile), then die or complete a run. 182
Alabaster Box
Achievement Alabaster Box.png
Alabaster Box
Alabaster Box has appeared in the basement Defeat The Lamb as Bethany. 183
Book Of Virtues
Achievement Book Of Virtues.png
Book Of Virtues
The Book of Virtues has appeared in the basement Defeat Isaac as Bethany. 184
Blessed Penny
Achievement Blessed Penny.png
Blessed Penny
The Blessed Penny has appeared in the basement Defeat ??? as Bethany. 185
Beth's Faith
Achievement Beths Faith.png
Beth's Faith
Beth's Faith has appeared in the basement Beat Boss Rush as Bethany. 186
Urn Of Souls
Achievement Urn Of Souls.png
Urn Of Souls
Urn Of Souls has appeared in the basement Defeat Satan as Bethany. 187
Rock Bottom
Achievement Rock Bottom.png
Rock Bottom
Rock Bottom has appeared in the basement Beat Boss Rush as Jacob & Esau. 188
Damocles
Achievement Damocles.png
Damocles
Damocles has appeared in the basement Defeat The Lamb as Jacob & Esau. 189
Jacob's Ladder
Achievement Jacob's Ladder.png
Jacob's Ladder
Jacob's Ladder has appeared in the basement Defeat Isaac as Jacob & Esau. 190
Birthright
Achievement Birthright.png
Birthright
Birthright has appeared in the basement Defeat ??? as Jacob & Esau. 191
Red Stew
Achievement Red Stew.png
Red Stew
Red Stew has appeared in the basement Defeat Satan as Jacob & Esau. 192
Meat Cleaver
Achievement Meat Cleaver.png
Meat Cleaver
Meat Cleaver has appeared in the basement Defeat The Witness as Isaac. 193
Yuck Heart
Achievement Yuck Heart.png
Yuck Heart
Yuck Heart has appeared in the basement Defeat The Witness as Magdalene. 194
Red Key
Achievement Red Key.png
Red Key
The Red Key has appeared in the basement Defeat The Witness as Cain. 195
Akeldama
Achievement Akeldama.png
Akeldama
Akeldama has appeared in the basement Defeat The Witness as Judas. 196
Eternal D6
Achievement Eternal D6.png
Eternal D6
Eternal D6 has appeared in the basement Defeat The Witness as ???. 197
Bird Cage
Achievement Bird Cage.png
Bird Cage
Bird Cage has appeared in the basement Defeat The Witness as Eve. 198
Donkey Jawbone
Achievement Donkeys Jawbone.png
Donkey Jawbone
A Donkey's Jawbone has appeared in the basement Defeat The Witness as Samson. 199
Devil's Crown
Achievement Devils Crown.png
Devil's Crown
Devil's Crown has appeared in the basement Defeat The Witness as Azazel. 200
Tinytoma
Achievement Tinytoma.png
Tinytoma
Tinytoma has appeared in the basement Defeat The Witness as Lazarus. 201
'M
Achievement M.png
'M
(garbled) has appeared in the basement Defeat The Witness as Eden. 202
Lost Soul
Achievement Lost Soul.png
Lost Soul
A Lost Soul has appeared in the basement Defeat The Witness as The Lost. 203
Revelation
Achievement Revelation.png
Revelation
Revelation has appeared in the basement Defeat The Witness as Bethany (currently bugged, cannot be unlocked). 204
Magic Skin
Achievement Magic Skin.png
Magic Skin
Magic Skin has appeared in the basement Defeat The Witness as Jacob & Esau. 205
Rune of Kenaz
Achievement Rune of Kenaz.png
Rune of Kenaz
The Rune of Kenaz has appeared in the basement Beat Challenge 2x: Scat Man. 206
Rune of Fehu
Achievement Rune of Fehu.png
Rune of Fehu
The Rune of Fehu has appeared in the basement Beat Challenge 3x: Turbo!. 207
Rune of Gebo
Achievement Rune of Gebo.png
Rune of Gebo
The Rune of Gebo has appeared in the basement Beat Challenge 4x: Bloody Mary. 208
Psy Fly
Achievement Psy Fly.png
Psy Fly
Psy fly has appeared in the basement Beat Challenge 8x: Isaac's Awakening (currently bugged, cannot be unlocked). 209
Rune of Othala
Achievement Rune of Othala.png
Rune of Othala
The Rune of Othala has appeared in the basement Beat Challenge 9x: Seeing Double. 210
Member Card
Achievement Member Card.png
Member Card
Member Card has appeared in the basement Unknown (Likely bugged). 211
Song of the Siren
Achievement Song of the Siren.png
Song of the Siren
Song of the Siren has appeared in the basement Bomb the skull left by The Siren after death. 212
Old Capacitor
Achievement Old Capacitor.png
Old Capacitor
Old Capacitor has appeared in the basement Blow up 10 charge machines. 213
Rotten Heart
Achievement Rotten Heart.png
Rotten Heart
Rotten Heart has appeared in the basement Enter the Corpse for the first time. 214
Mega Mush
Achievement Mega Mush.png
Mega Mush
Mega Mush has appeared in the basement Defeat The Witness with every character on Hard mode. 215
Challenge #3x
Achievement 3x.png
Challenge #3x
You unlocked Challenge #3x: Turbo! Unlock Cain. 216
Challenge #4x
Achievement 4x.png
Challenge #4x
You unlocked Challenge #4x: Bloody Mary Unlock Bethany, It Lives and Blood Bag. 217
Challenge #8x
Achievement 8x.png
Challenge #8x
You unlocked Challenge #8x: Isaac's Awakening Defeat The Witness. 218
Challenge #9x
Achievement 9x.png
Challenge #9x
You unlocked Challenge #9x: Seeing Double Defeat The Witness. 219
Soul Baby
Achievement Soul Baby.png
Soul Baby
You unlocked Soul Baby Defeat Mom's Heart or It Lives as Bethany on Hard Mode. 220
Double Baby
Achievement Double Baby.png
Double Baby
You unlocked Double Baby Defeat Mom's Heart or It Lives as Jacob & Esau on Hard Mode. 221

Sacdag.png Antibirth Sacdag.png

Boss Baby Plum.png Bosses MainPageChallenges.png Challenges MainPageChapters.png Chapters Character.png Characters Book of Virtues.png Items
Dumpling.png Monsters Bucket.png Objects Immortal Heart.png Pickups Blessed Penny Icon.png Trinkets MainPageAchievements.png Unlockables
Community content is available under CC BY-NC-SA 3.0 unless otherwise noted.