Unlockables

Unlockables are new content for the game such as items, characters, or challenges that must be unlocked. They pop up when something has been completed or achieved.

Name	Image	Description	Unlock	In Game Secret Number
Bethany		You unlocked Bethany	Have any combination of 12 soul, black, or immortal hearts during a run	179
Jacob & Esau		You unlocked Jacob & Esau.	Defeat The Witness	180
Planetarium		The stars are calling...	Acquire three astrology-related items in one run.	181
A Secret Exit		Your fate diverges	Defeat the three new bosses found on normal floors (Baby Plum, Reap Creep, and The Pile), then die or complete a run.	182
Alabaster Box		Alabaster Box has appeared in the basement	Defeat The Lamb as Bethany.	183
Book Of Virtues		The Book of Virtues has appeared in the basement	Defeat Isaac as Bethany.	184
Blessed Penny		The Blessed Penny has appeared in the basement	Defeat ??? as Bethany.	185
Beth's Faith		Beth's Faith has appeared in the basement	Beat Boss Rush as Bethany.	186
Urn Of Souls		Urn Of Souls has appeared in the basement	Defeat Satan as Bethany.	187
Rock Bottom		Rock Bottom has appeared in the basement	Beat Boss Rush as Jacob & Esau.	188
Damocles		Damocles has appeared in the basement	Defeat The Lamb as Jacob & Esau.	189
Jacob's Ladder		Jacob's Ladder has appeared in the basement	Defeat Isaac as Jacob & Esau.	190
Birthright		Birthright has appeared in the basement	Defeat ??? as Jacob & Esau.	191
Red Stew		Red Stew has appeared in the basement	Defeat Satan as Jacob & Esau.	192
Meat Cleaver		Meat Cleaver has appeared in the basement	Defeat The Witness as Isaac.	193
Yuck Heart		Yuck Heart has appeared in the basement	Defeat The Witness as Magdalene.	194
Red Key		The Red Key has appeared in the basement	Defeat The Witness as Cain.	195
Akeldama		Akeldama has appeared in the basement	Defeat The Witness as Judas.	196
Eternal D6		Eternal D6 has appeared in the basement	Defeat The Witness as ???.	197
Bird Cage		Bird Cage has appeared in the basement	Defeat The Witness as Eve.	198
Donkey Jawbone		A Donkey's Jawbone has appeared in the basement	Defeat The Witness as Samson.	199
Devil's Crown		Devil's Crown has appeared in the basement	Defeat The Witness as Azazel.	200
Tinytoma		Tinytoma has appeared in the basement	Defeat The Witness as Lazarus.	201
'M		(garbled) has appeared in the basement	Defeat The Witness as Eden.	202
Lost Soul		A Lost Soul has appeared in the basement	Defeat The Witness as The Lost.	203
Revelation		Revelation has appeared in the basement	Defeat The Witness as Bethany (currently bugged, cannot be unlocked).	204
Magic Skin		Magic Skin has appeared in the basement	Defeat The Witness as Jacob & Esau.	205
Rune of Kenaz		The Rune of Kenaz has appeared in the basement	Beat Challenge 2x: Scat Man.	206
Rune of Fehu		The Rune of Fehu has appeared in the basement	Beat Challenge 3x: Turbo!.	207
Rune of Gebo		The Rune of Gebo has appeared in the basement	Beat Challenge 4x: Bloody Mary.	208
Psy Fly		Psy fly has appeared in the basement	Beat Challenge 8x: Isaac's Awakening (currently bugged, cannot be unlocked).	209
Rune of Othala		The Rune of Othala has appeared in the basement	Beat Challenge 9x: Seeing Double.	210
Member Card		Member Card has appeared in the basement	Unknown (Likely bugged).	211
Song of the Siren		Song of the Siren has appeared in the basement	Bomb the skull left by The Siren after death.	212
Old Capacitor		Old Capacitor has appeared in the basement	Blow up 10 charge machines.	213
Rotten Heart		Rotten Heart has appeared in the basement	Enter the Corpse for the first time.	214
Mega Mush		Mega Mush has appeared in the basement	Defeat The Witness with every character on Hard mode.	215
Challenge #3x		You unlocked Challenge #3x: Turbo!	Unlock Cain.	216
Challenge #4x		You unlocked Challenge #4x: Bloody Mary	Unlock Bethany, It Lives and Blood Bag.	217
Challenge #8x		You unlocked Challenge #8x: Isaac's Awakening	Defeat The Witness.	218
Challenge #9x		You unlocked Challenge #9x: Seeing Double	Defeat The Witness.	219
Soul Baby		You unlocked Soul Baby	Defeat Mom's Heart or It Lives as Bethany on Hard Mode.	220
Double Baby		You unlocked Double Baby	Defeat Mom's Heart or It Lives as Jacob & Esau on Hard Mode.	221