Challenges

Challenges are unique runs that typically start the player off with one or more specific items or modifies game mechanics to make the game more difficult. Completing challenges unlocks new content for the game. Special Seeds occupy and create similar effects and runs.

Challenge list

Name	Conditions	Goal	Unlocked by	Unlocks
1x. N/A
2x. Scat Man	Starts with: Skatole, 9 Volt, Butt Bombs, E Coli, BFFS!, Thunder Thighs, Dirty Mind, and Mysterious Candy. Blindfolded (cannot use tears). Damage must be primarily done with the friendly Dips spawned by Dirty Mind. No treasure rooms.	Mom	Unlocked from start	Rune of Kenaz
3x. Turbo!	This challenge is done as Cain. The game is massively sped up.	Mom	Unlock Cain	Rune of Fehu
4x. Bloody Mary	This challenge is done as Bethany. Starts with: Blood Bag, Anemic, Book of Belial, Child's Heart, and Blood Oath. No treasure rooms.	Satan	Unlock Bethany, It Lives, and Blood Bag	Rune of Gebo
5x. N/A
6x. N/A
7x. N/A
8x. Isaac's Awakening	Starts with: Trinity Shield, Mom's Bracelet, and Spirit Sword. No treasure rooms.	The Witness	Defeat The Witness	Temporarily bugged - does not unlock a reward on completion (supposed to unlock Psy Fly)
9x. Seeing Double	This challenge is done as Jacob & Esau. Jacob starts with 20/20. Esau starts with 20/20 and X-Ray Vision. All enemies (including bosses) are doubled. An enemy's double can be an alternate version, such as a champion. Enemies in challenge rooms are not doubled (possibly a bug).	It Lives	Defeat The Witness	Rune of Othala
10x. N/A

Challenges
1x.	Coming soon	2x.	Scat Man	3x.	Turbo!	4x.	Bloody Mary	5x.	Coming soon
6x.	Coming soon	7x.	Coming soon	8x.	Isaac's Awakening	9x.	Seeing Double	10x.	Coming soon